Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Lipase from Aspergillus oryzae >=20,000 U/g | 9001-62-1 | MFCD03457337 | 250ML
Lipase from Aspergillus oryzae >=20,000 U/g | 9001-62-1 | MFCD03457337 | 250ML
Sigma Aldrich Fine Chemicals Biosciences Cellulase from Aspergillus niger | 9012-54-8 | MFCD00081510 | 100ku
Cellulase from Aspergillus niger | 9012-54-8 | MFCD00081510 | 100ku
Sigma Aldrich Fine Chemicals Biosciences α-Amylase from Bacillus sp. powder, yellow-brown, ~50 U/mg | 9000-90-2 | MFCD00081319 | 10g
α-Amylase from Bacillus sp. powder, yellow-brown, ~50 U/mg | 9000-90-2 | MFCD00081319 | 10g
LGC GENOMICS LLC (Lucigen) Exonuclease III, 25,000 U @ 200 U/LDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3-recessed end, producing stretches of ssDNA.
Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' endsProtect protruding 3' ends because this exonuclease will not start digestion at protruding 3' overhangsThis product includes both Exonuclease III enzyme and a 10X reaction buffer.
Takara Bio RNASE A 100MG
Rnasea (Lyophilized) 100Mg. The Rnase A Is Primarily Intended To Be Used With Nucleospin And Nucleobond Kits.
Enzo Life Sciences Matrix metalloproteinase (MMP) multipack-2 (1 Kit)
The MMP multipack-2 contains 10µg each of five highly active recombinant MMP catalytic domains: MMP-3, MMP-7, MMP-10, MMP-11, and MMP-12. Contents: Contains 10µg of each enzyme, provided in a screw-cap microfuge tube. MMP-3 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE109), MMP-7 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE181), MMP-10 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE392), MMP-11 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE282), MMP-12 (catalytic domain) (human), (recombinant) (Prod. No. BML-SE138). Application: FUNC, HTS. Long Term Storage: -80°C
New England Biolabs, Inc. T4 DNA Ligase – 100,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. BspQI – 2500 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
New England Biolabs, Inc. PvuII-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence CAG^CTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. T4 Polynucleotide Kinase – 500 units
T4 PNK catalyzes the transfer and exchange of P from the gamma; position of ATP to the 5 -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3-phosphoryl groups from 3-phosphoryl polynucleotides, deoxynucleoside 3-monophosphates and deoxynucleoside 3-diphosphates.
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- Removal of 3' phosphoryl groups
New England Biolabs, Inc. T7 RNA Polymerase – 25000 units
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. T4 DNA Ligase – 20,000 units – 400,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 2000 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
New England Biolabs, Inc. T3 DNA Ligase – 750000 units
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.